Paternal age and sperm DNA decay: discrepancy between chromomycin and aniline blue staining
نویسندگان
چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملevaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
متن کاملChromomycin A3 Staining Indicates Sperm Protamine Deficiency
Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.
متن کاملThe utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis
OBJECTIVE The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. METHODS Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. RESULTS Prevalence of strict morphology of less than...
متن کاملthe relationship between listening self efficacy, listening comprehension, gende, and age in male and female english learners
چکیده هدف اصلی تحقیق حاضر مطالعه و بررسی رابطه بین خودکارامدی شنیداری با درک شنیداری بود.همچنین رابطه درک شنیداری وخودکارآمدی شنیداری با جنسیت و رابطه خودکارامدی شنیداری با سن نیز مورد بررسی قرار گرفت.تعداد شصت زبان اموز-سی دختر و سی پسر- کانون زبان ایران در مرکز خرم آباد به صورت تصادفی انتخاب شدند. روشهای به کار رفته در جمع اوری داده ها عبارت بودند ازبرسشنامه خودکارآمدی و تست درک شنیداری لانگ...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)60083-1